Performance of Single and Two-Stage Cross-Flow Ultrafiltration Membrane in Fractionation of Peptide from Microalgae Protein Hydrolysate (Nannochloropsis gaditana)

Cross-flow ultrafiltration (UF) membrane with two different configurations; single (10 kDa and 5 kDa) two-stage (10/5 in fractionating microalgae protein hydrolysate (MPH) were studied to obtain a low molecular weight of peptide. The effect flow rate, trans-membrane pressure (TMP), pH MPH evaluated based on permeate flux peptide transmission. results showed that, for UF membrane, optimum operating parameters at rate 23 mL/min, TMP 1.5 bar, 9, 43.65 L/m2 h 55.42 (5 transmission 58.20% 67.34% kDa). Meanwhile, the best observed 2, 69.85 79.13%. Fractionation was be better producing compared membrane. In conclusion, it possible produce high amount by controlling suitable configuration